Alkalotolerance of Yersinia enterocolitica as a basis for selective isolation from food enrichments

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Isolation of Yersinia enterocolitica from swine.

Data pertaining to 20 strains of Yensinia enterocolitica isolated from the cecal content of swine slaughtered at Toronto Abattoirs are presented. Fifteen of 20 cultures belonged to the two predominant human serotypes in Ontario (i.e., serotype O:3 and O:5,27.) Seven cultures of Y. enterocolitica O:3 belonged to the "Canadian" human phage type 9b. These findings are further proof that swine are ...

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Direct isolation of Yersinia enterocolitica and Yersinia pseudotuberculosis from meat.

Studies were done to determine the usefulness of dilute alkali (KOH) treatment of meat samples for direct isolation of Yersinia enterocolitica and Yersinia pseudotuberculosis, without enrichment. Virulent Y. enterocolitica and Y. pseudotuberculosis in pork contaminated with 10(2), 10(3), and 10(4) cells per g survived the direct KOH treatment and were never recovered by using KOH postenrichment...

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Differential and selective medium for isolation of Yersinia enterocolitica from stools.

A new differential and selective medium, DYS agar, was developed and evaluated from the isolation of Yersinia enterocolitica. Ther bile salts content of the medium resulted in high selectivity, and inclusion of arabinose, lysine, and arginine rendered Y. Enterocolitica very distinct from Proteus spp., Pseudomonas spp., and other members of the family Enterobacteriaceae. DYS medium was more effi...

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Comparison of Enrichment Schemes for the Isolation of Yersinia Enterocolitica

Discussion: Considering the effect of sample weight on the detection of Salmonella bacteria (Davies et al., 2000), we cannot directly compare the three enrichment media used herein. However, we suggest the use of M1 whenever possible to obtain large amounts of fecal material (10g). Furthermore, the use of direct enrichment of swine feces in SC is discouraged. The coupling of RV to PCR was signi...

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Yersinia enterocolitica as a cause of septicemia in crucian carp (Carassius carassius)

Yersinia enterocolitica is an important pathogen to animals and humans. Thirteen strains of Y. enterocolitica were isolated from diseased fish. The partial 16SrDNA gene, five virulence genes, pathogenicity, and drug resistance of Y. enterocolitica strain G6029 were studied using molecular biological technology and toxicological method. Results showed that the length of amplified 16SrDNA sequenc...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1983

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.46.1.22-27.1983